Innovative Chromatography Products
www.restek.fr
www.restek.fr
Fentanyl and Analogues in Urine on Raptor Biphenyl by LC-MS/MS
| Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
|---|---|---|---|---|---|---|
| 1. | Norfentanyl-d5 | 1.03 | 1 | 238.30 | 84.15 | - |
| 2. | Norfentanyl | 1.04 | 10 | 233.27 | 84.15 | 56.06 |
| 3. | Remifentanil | 1.34 | 10 | 377.37 | 113.15 | 317.30 |
| 4. | Acetyl fentanyl-13C6 | 1.69 | 1 | 329.37 | 188.25 | - |
| 5. | Acetyl fentanyl | 1.69 | 10 | 323.37 | 188.25 | 105.15 |
| 6. | Alfentanil | 1.88 | 10 | 417.47 | 268.31 | 197.23 |
| 7. | Fentanyl-d5 | 1.95 | 1 | 342.47 | 188.27 | - |
| 8. | Fentanyl | 1.96 | 10 | 337.37 | 188.26 | 105.08 |
| 9. | Carfentanil-d5 | 2.04 | 1 | 400.40 | 340.41 | - |
| 10. | Carfentanil | 2.05 | 10 | 395.40 | 113.14 | 335.35 |
| 11. | Butyryl fentanyl | 2.15 | 10 | 351.43 | 188.20 | 105.15 |
| 12. | Sufentanil-d5 | 2.25 | 1 | 392.40 | 238.25 | - |
| 13. | Sufentanil | 2.26 | 10 | 387.40 | 238.19 | 111.06 |
LC_CF0715
| Column | Raptor Biphenyl (cat.# 9309552) | ||||||||||||||||||||
| Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
| Particle Size: | 5 µm | ||||||||||||||||||||
| Pore Size: | 90 Å | ||||||||||||||||||||
| Guard Column: | Raptor Biphenyl EXP guard cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252) | ||||||||||||||||||||
| Temp.: | 40 °C | ||||||||||||||||||||
| Sample | |||||||||||||||||||||
| Diluent: | 70:30 Water:methanol | ||||||||||||||||||||
| Inj. Vol.: | 5 µL | ||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||
| A: | 0.1% Formic acid in water | ||||||||||||||||||||
| B: | 0.1% Formic acid in methanol | ||||||||||||||||||||
| |||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||
| Instrument | UHPLC | ||||||||||||||||||||
| Notes | Human urine was fortified at 10 ng/mL with target analytes. An 80 μL urine aliquot was mixed with 320 μL of 70:30 water:methanol solution (5-fold dilution) and 10 μL of internal standard solution (40 ng/mL in methanol) in a Thomson SINGLE StEP filter vial (Restek cat.# 25895). After filtering through the 0.2 µm PVDF membrane, 5 μL was injected for analysis. |
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