Innovative Chromatography Products
www.restek.fr
www.restek.fr
Methylmalonic Acid in SeraFlx BIOMATRIX on Force C18 by LC-MS/MS
| Peaks | tR (min) | Precursor Ion | Product Ion | |
|---|---|---|---|---|
| 1. | Succinic acid | 1.69 | 117.3 | 73.1 |
| 2. | Methyl-D3-malonic acid | 2.02 | 120.2 | 76.2 |
| 3. | Methylmalonic acid | 2.03 | 117.3 | 73.1 |
LC_CF0717
| Column | Force C18 (cat.# 963431E) | ||||||||||||||||||||||||
| Dimensions: | 100 mm x 3.0 mm ID | ||||||||||||||||||||||||
| Particle Size: | 3 µm | ||||||||||||||||||||||||
| Pore Size: | 100 Å | ||||||||||||||||||||||||
| Guard Column: | Force C18 EXP guard column cartridge 3 mm ID, (cat.# 963450253) | ||||||||||||||||||||||||
| Temp.: | 35 °C | ||||||||||||||||||||||||
| Sample | |||||||||||||||||||||||||
| Conc.: | 50 ng/mL methylmalonic acid in SeraFlx BIOMATRIX | ||||||||||||||||||||||||
| Inj. Vol.: | 3 µL | ||||||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||||||
| A: | 0.5% Formic acid in water | ||||||||||||||||||||||||
| B: | 0.5% Formic acid in methanol | ||||||||||||||||||||||||
| |||||||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||||||
| Ion Mode: | ESI- | ||||||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||||||
| Instrument | HPLC | ||||||||||||||||||||||||
| Notes | 100 μL of sample (SeraFlx BIOMATRIX) was aliquoted for extraction. The sample was fortified at 50 ng/mL with methylmalonic acid. It was also fortified with 5 μL of succinic acid (20 μg/mL in water, for validation purposes only). 5 μL of the internal standard (2,500 ng/mL MMA-D3 in water) was added to the sample. The sample was precipitated using 300 μL of 0.5% formic acid in methanol followed by a 10 second vortex at 3,000 rpm. The sample was then centrifuged at 4,000 rpm for 10 minutes at 10 °C. 250 μL of the supernatant was filtered using a Thomson SINGLE StEP standard filter vial (PVDF, 0.2 μm, Restek cat.# 25895) prior to analysis. |
RECHERCHES SIMILAIRES
Pour optimiser l'impression de votre chromatogramme, pensez à bien paramétrer l'imprimante !
Dans la fenêtre « Propriétés de l’imprimante » :
1- Choisissez « Ajuster au format de la page »
2- Sélectionner le format « Paysage » pour les chromatogrammes aux nombreux composés,
« Portrait » pour les chromatogrammes aux pics les plus hauts.
