Innovative Chromatography Products
www.restek.fr
www.restek.fr
EtG/EtS in UTAK EtG Plus Level 1 Urine Control on Raptor EtG/EtS by LC-MS/MS
| Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
|---|---|---|---|---|---|---|
| 1. | Ethyl-β-D-glucuronide | 0.81 | 500 | 221.20 | 75.1 | 85.1 |
| 2. | Ethyl-β-D-glucuronide-d5 | 0.81 | 100 | 226.20 | 85.00 | - |
| 3. | Ethyl sulfate-d5 | 1.84 | 100 | 130.10 | 98.05 | - |
| 4. | Ethyl sulfate | 1.87 | 200 | 125.10 | 97.10 | 80.00 |
LC_CF0726
| Column | Raptor EtG/EtS (cat.# 9325A12) | ||||||||||||||||||||
| Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
| Particle Size: | 2.7 µm | ||||||||||||||||||||
| Pore Size: | 90 Å | ||||||||||||||||||||
| Guard Column: | UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809) | ||||||||||||||||||||
| Temp.: | 35 °C | ||||||||||||||||||||
| Sample | |||||||||||||||||||||
| Ethyl- | |||||||||||||||||||||
| Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104) | |||||||||||||||||||||
| Diluent: | 0.1% Formic acid in water | ||||||||||||||||||||
| Conc.: | UTAK EtG Plus urine quality control (200 ng/mL EtS and 500 ng/mL EtG) | ||||||||||||||||||||
| Inj. Vol.: | 10 µL | ||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||
| A: | 0.1% Formic acid in water | ||||||||||||||||||||
| B: | 0.1% Formic acid in acetonitrile | ||||||||||||||||||||
| |||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||
| Ion Source: | Electrospray | ||||||||||||||||||||
| Ion Mode: | ESI- | ||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||
| Instrument | HPLC | ||||||||||||||||||||
| Notes | UTAK EtG Plus Level 1 control contains EtG at 500 ng/mL and EtS at 200 ng/mL concentrations. A 50 μL aliqout was taken from the the Level 1 control and diluted 20 times with 950 μL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C and 10 μL was injected for LC-MS/MS analysis. |
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