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EtG/EtS in UTAK EtG Plus Level 1 Urine Control on Raptor EtG/EtS by LC-MS/MS

Column | Raptor EtG/EtS (cat.# 9325A12) | ||||||||||||||||||||
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809) | ||||||||||||||||||||
Temp.: | 35 °C | ||||||||||||||||||||
Sample | |||||||||||||||||||||
Ethyl- | |||||||||||||||||||||
Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104) | |||||||||||||||||||||
Diluent: | 0.1% Formic acid in water | ||||||||||||||||||||
Conc.: | UTAK EtG Plus urine quality control (200 ng/mL EtS and 500 ng/mL EtG) | ||||||||||||||||||||
Inj. Vol.: | 10 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||
B: | 0.1% Formic acid in acetonitrile | ||||||||||||||||||||
| |||||||||||||||||||||
Detector | MS/MS | ||||||||||||||||||||
Ion Source: | Electrospray | ||||||||||||||||||||
Ion Mode: | ESI- | ||||||||||||||||||||
Mode: | MRM | ||||||||||||||||||||
Instrument | HPLC | ||||||||||||||||||||
Notes | UTAK EtG Plus Level 1 control contains EtG at 500 ng/mL and EtS at 200 ng/mL concentrations. A 50 μL aliqout was taken from the the Level 1 control and diluted 20 times with 950 μL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C and 10 μL was injected for LC-MS/MS analysis. |
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