Pesticide Residue Analysis in Kale by LC-MS/MS on Ultra Aqueous C18

PeakstR (min)MRM 1MRM 2
1.Methamidophos1.27142.1 / 94.1142.1 / 112.2
2.Acephate1.78184.1 / 125.1184.1 / 95.1
3.Propamocarb2.65189.2 / 102.0189.2 / 144.2
4.Omethoate2.94214.1 / 125.2214.1 / 155.1
5.Aldicarb sulfone3.45223.2 / 148.0223.2 / 76.2
6.Aldicarb sulfoxide3.71207.2 / 132.1207.2 / 89.1
7.Pymetrozine3.82218.1 / 105.0218.1 / 78.2
8.Oxamyl3.90237.1 / 71.9237.1 / 90.1
9.Methomyl4.02163.1 / 88.1163.1 / 106.2
10.Monocrotophos4.38224.1 / 127.1224.1 / 98.1
11.Dimethoate4.81230.1 / 125.2230.1 / 171.2
12.Mevinphos E4.99225.2 / 193.3225.2 / 127.2
13.Thiabendazole4.99202.2 / 175.0202.2 / 131.2
14.Imidacloprid5.02256.3 / 209.1256.3 / 175.2
15.Mevinphos Z5.43225.1 / 193.2225.1 / 127.1
16.Aldicarb5.55208.2 / 116.2208.2 / 89.1
17.Carbetamide5.73237.1 / 192.0237.1 / 118.1
18.Imazethapyr5.90290.1 / 245.2290.1 / 177.3
19.Thidiazuron5.93221.2 / 102.0221.2 / 128.1
20.Thiophanate methyl5.96343.2 / 151.1343.2 / 93.1
21.Propoxur5.99210.2 / 168.1210.2 / 111.2
22.Bendiocarb6.00224.1 / 109.2224.1 / 167.2
23.Dichlorvos6.01220.9 / 109.2220.9 / 95.0
24.Carbofuran6.09222.3 / 165.2222.3 / 123.1
25.Pirimicarb6.15239.2 / 72.2239.2 / 182.2
26.Carbaryl6.32202.3 / 127.1202.3 / 117.2
27.Imazalil6.40297.1 / 159.0297.1 / 173.1
28.Isoprocarb6.53194.3 / 95.2194.3 / 137.3
29.Metalaxyl6.72280.4 / 192.3280.4 / 160.2
30.Metalaxyl-m6.72280.4 / 220.3280.4 / 192.1
31.Atrazine6.75216.2 / 174.3216.2 / 132.1
32.Atrazine-d5 (IS)6.77221.1 / 179.0221.1 / 101.2
33.Isoproturon6.86207.2 / 72.3207.2 / 134.3
34.Azinphos-methyl6.96318.2 / 160.0318.2 / 132.1
35.Diuron6.97233.1 / 72.1233.1 / 160.0
36.Phosmet6.97318.1 / 160.2318.1 / 133.0
37.Demeton-O6.98259.0 / 89.1259.0 / 61.2
38.Demeton-S6.98259.2 / 88.9259.2 / 61.1
39.Nuarimol7.08315.0 / 252.1315.0 / 81.0
40.Propanil7.11218.2 / 162.0218.2 / 127.0
41.Azoxystrobin7.12404.3 / 372.3404.3 / 344.0
42.Malathion7.13331.1 / 127.1331.1 / 99.1
43.Methiocarb7.14226.1 / 169.1226.1 / 121.1
44.Chlorpropham7.20214.1 / 154.0214.1 / 126.1
45.Linuron7.20249.2 / 160.1249.2 / 182.1
46.Crotoxyphos7.21332.2 / 211.2332.2 / 167.2
47.Promecarb7.21208.8 / 109.2208.8 / 151.3
48.Propetamphos7.22282.1 / 138.0282.1 / 110.2
49.Boscalid7.25343.2 / 307.2343.2 / 140.0
50.Triadimefon7.26294.3 / 197.1294.3 / 69.2
51.Triadimenol7.35296.3 / 70.2296.3 / 227.1
52.Fenhexamid7.40302.1 / 97.1302.1 / 55.0
53.Myclobutanil7.43289.3 / 70.2289.3 / 124.9
54.Dichlofluanid7.44332.9 / 224.0332.9 / 123.1
55.Triazophos7.48314.1 / 162.0314.1 / 119.2
56.Alachlor7.60270.2 / 238.1270.2 / 162.2
57.Fenarimol7.61331.0 / 268.0331.0 / 81.0
58.Iprodione7.66330.3 / 245.2332.3 / 247.0
59.Ethoprop7.70243.1 / 131.0243.1 / 173.0
60.Parathion7.77292.1 / 236.0292.1 / 140.1
61.Fenamiphos7.83304.4 / 217.2304.4 / 202.0
62.Diflubenzuron7.85311.1 / 158.2311.1 / 141.1
63.Fenoxycarb7.87302.1 / 88.0302.1 / 116.1
64.Etaconazole isomer 17.89328.2 / 159.1328.2 / 123.0
65.Fenbuconazole7.89337.3 / 125.3337.3 / 70.3
66.Kresoxim-methyl7.90314.2 / 115.9314.2 / 131.0
67.Tolyfluanid8.00364.0 / 238.0364.0 / 137.1
68.Etaconazole isomer 28.01328.3 / 159.2328.3 / 123.1
69.Fenthion8.06279.1 / 169.1279.1 / 105.1
70.Quinalphos8.07299.3 / 243.1299.3 / 163.2
71.Cyprodinil8.16226.1 / 93.3226.1 / 77.1
72.Tebuconazole8.24308.3 / 70.1308.3 / 125.1
73.Chlorfenvinphos8.32359.2 / 155.1359.2 / 99.2
74.Diazinon8.34305.2 / 169.3305.2 / 153.1
75.Pirimiphos methyl8.35306.1 / 164.3306.1 / 108.1
76.Phosalone8.38368.1 / 182.1368.1 / 138.0
77.Diazinon-d10 (IS)8.39315.3 / 170.1315.3 / 154.1
78.Coumaphos8.46363.1 / 227.2363.1 / 211.1
79.Propiconazole isomer 18.60342.3 / 159.0342.3 / 69.3
80.Pyraclostrobin8.61388.0 / 164.2388.0 / 194.3
81.Chlorpyrifos methyl8.72323.9 / 125.0323.9 / 291.8
82.Propiconazole isomer 28.77342.4 / 159.1342.4 / 69.4
83.Dialifos8.81394.3 / 208.0394.3 / 187.0
84.Prochloraz9.01376.1 / 308.1376.1 / 266.0
85.Indoxacarb9.04528.6 / 218.0528.6 / 150.2
86.Trifloxystrobin9.18409.4 / 186.0409.4 / 145.1
87.Spinosyn A9.58733.1 / 142.4733.1 / 98.4
88.Difenoconazole isomer 19.60406.3 / 251.1408.2 / 253.1
89.Triflumizole9.79346.2 / 278.2346.2 / 73.1
90.Difenoconazole isomer 29.85406.4 / 251.2408.3 / 253.2
91.Ethion10.20385.3 / 199.0385.3 / 171.0
92.Spinosyn D10.38746.8 / 142.4746.8 / 98.3
93.Chlorpyrifos10.40350.0 / 198.0350.0 / 97.0
94.Pendimethalin10.47282.3 / 212.2282.3 / 194.3
95.Emamectin B1a benzoate10.48887.2 / 158.3887.2 / 126.3
96.Propargite10.61368.4 / 175.1368.4 / 231.2
97.Fenpropathrin10.67350.3 / 125.0350.3 / 97.4
98.Flufenoxuron10.72489.5 / 158.2489.5 / 141.1
99.Lambda cyhalothrin10.72467.4 / 225.1467.4 / 181.0
100.Deltamethrin10.78523.3 / 280.9523.3 / 181.0
101.trans-Permethrin10.90408.4 / 183.3408.4 / 153.2
102.Leptophos10.92413.2 / 171.0413.2 / 77.1
103.cis-Permethrin10.95408.5 / 183.1408.5 / 153.2
104.Bifenthrin10.98440.3 / 181.2440.3 / 166.2
Isomers were designated 1 or 2 by elution order
image
LC_FF0516
ColumnUltra Aqueous C18 (cat.# 9178312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:50 °C
SampleKale extract diluted 10x in mobile phase A
Diluent:Mobile phase A
Conc.:10 ng/mL
Inj. Vol.:20 µL
Mobile Phase
A:Water + 0.1% formic acid + 4 mM ammonium formate
B:Methanol + 0.1% formic acid + 4 mM ammonium formate
Time (min)Flow (mL/min)%A%B
0.000.59010
1.500.59010
6.000.53070
9.000.53070
10.000.50100
12.000.50100
12.010.59010
15.000.59010
DetectorABSCIEX MS/MS
Model #:API 4000
Ion Source:TurboIonSpray®
Ion Mode:ESI+
Ion Spray Voltage:5.5 kV
Curtain Gas:30 psi (206.8 kPa)
Gas 1:40 psi (275.8 kPa)
Gas 2:45 psi (310.3 kPa)
CAD:10 psi (68.9 kPa)
Source Temp.:450 °C
Mode:Scheduled MRM
MRM Detection Window:45 sec
Target Scan Time:0.33 sec
InstrumentAPI LC-MS/MS
NotesThe AOAC QuEChERS method was used. 15 mL of acetonitrile with 1% acetic acid (v/v) was added to 15 g of fortified homogenized kale. Q-sep AOAC buffering extraction salts (cat.# 26237) containing 6 g MgSO4 and 1.5 g sodium acetate were added. Following 1 minute of manual shaking, samples were centrifuged for 5 minutes at 3,000 U/min with a Q-sep 3000 centrifuge (cat.# 26230). The top acetonitrile layer was removed to a clean vial. A Restek Q-sep dSPE tube, cat.# 26126, containing 300 mg PSA, 150 mg GCB, and 900 mg MgSO4 was used to process 6 mL of kale extract. The tube was shaken for 2 minutes and centrifuged for 5 minutes at 3,000 U/min. The sample was diluted 10-fold in mobile phase A before injection.
Voir en PDF

RECHERCHES SIMILAIRES

ultra

,

ultra aqueous

,

C18

,

food

,

kale

,

MS/MS

,

HPLC

,

electrospray

,

turbospray

,

ESI+

,

triplequad

,

QuEChERS

,

LC-MS/MS

,

dSPE

,

dispersive SPE

,

pesticides

Pour optimiser l'impression de votre chromatogramme, pensez à bien paramétrer l'imprimante !
Dans la fenêtre « Propriétés de l’imprimante » :
1- Choisissez « Ajuster au format de la page »
2- Sélectionner le format « Paysage » pour les chromatogrammes aux nombreux composés,
« Portrait » pour les chromatogrammes aux pics les plus hauts.


Restek Domestic Customer Service

Objet

Votre message

Nom et Prénom

Adresse e-mail

Votre société

Adresse de l'entreprise

Spam Block (Please leave this blank)
Glissez-déposez vos pièces jointes ici (optionnel)
...

Tous les champs sont requis

Merci.

Votre message a bien été envoyé. Nous vous recontacterons dans les plus brefs délais.

Message non envoyé.

Votre message ne peut pas être envoyé pour le moment, veuillez nous excuser pour la gêne occasionnée. Réessayez plus tard ou contactez Restek au 01 60 72 32 10.

www.restek.com/Contact-Us