Innovative Chromatography Products
www.restek.fr
www.restek.fr
Cannabis Flower Sample on Raptor ARC-18 2.7 μm by HPLC-UV
| Peaks | tR (min) | |
|---|---|---|
| 1. | Cannabidivarinic acid (CBDVA) | 2.72 |
| 2. | Cannabigerolic acid (CBGA) | 2.90 |
| Peaks | tR (min) | |
|---|---|---|
| 3. | Tetrahydrocannabivarinic acid (THCVA) | 4.45 |
| 4. | Δ9-Tetrahydrocannabinol (Δ9-THC) | 6.05 |
| 5. | Tetrahydrocannabinolic acid A (THCA-A) | 7.87 |
LC_GN0588
| Column | Raptor ARC-18 (cat.# 9314A65) | ||||||||||||
| Dimensions: | 150 mm x 4.6 mm ID | ||||||||||||
| Particle Size: | 2.7 µm | ||||||||||||
| Pore Size: | 90 Å | ||||||||||||
| Guard Column: | Raptor ARC-18 EXP guard column cartridge 2.7 µm (cat.# 9314A0250) | ||||||||||||
| Temp.: | 30 °C | ||||||||||||
| Sample | |||||||||||||
| Diluent: | 25:75 Water:methanol | ||||||||||||
| Inj. Vol.: | 5 µL | ||||||||||||
| Mobile Phase | |||||||||||||
| A: | Water, 5 mM ammonium formate, 0.1% formic acid | ||||||||||||
| B: | Acetonitrile, 0.1% formic acid | ||||||||||||
| |||||||||||||
| Detector | UV/Vis @ 228 nm | ||||||||||||
| Instrument | HPLC | ||||||||||||
| Notes | 200 mg of ground flower was weighed into a 50 mL centrifuge tube. 10 mL of IPA was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis. |
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